Isolation and Identification of Strain with D-aminoacylase Activity: Preliminary Characterization of the Enzyme
نویسنده
چکیده
The results of BLAST analyses of partial nucleotide sequence of 16S ribosomal RNA gene of newly isolated strain N 6.1, possessing D-aminoacylase activity, revealed maximum of 83 % identity with Bacterial and Archaeal databases, indicating that the isolated bacteria belongs to a new species and even the new genus. The enzyme was more than 850 times purified with 2.7 % overall yield as a result of 4 stage purification scheme. The purified D-aminoacylase showed optimal activity at 40C and pH 7.5-7.8. The enzyme retained 50 % of its initial activity when incubated 20 min at 40C, and in these conditions it was more stable at pH 8.5-9.5.
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